• Background

  Leptospirosis is considered the most widespread zoonotic disease in the world with a notable (re-)emergence during the past decade. The World Health Organization (WHO) recognizes leptospirosis outbreaks to be driven by climate and environment since warmer and wetter conditions favor the spread of the infection. Leptospira have a broad host range and leptospirosis may present with a broad spectrum of clinical symptoms.

  While maintenance hosts like rats generally remain asymptomatic, in humans, who are dead-end hosts, clinical manifestations are highly variable ranging from mild influenza-like symptoms to severe multisystemic illness with potentially fatal outcome. At-risk population include farmers and agricultural workers, fishermen, pest exterminators, water sports people, sanitary workers, sewage workers, animal caretakers, abattoir staff. In livestock, the clinical signs of leptospirosis include reduced fertility, stillbirth and abortion. Acute disease in dogs may present with acute kidney injury and liver impairment and respiratory, sometimes also gastrointestinal, signs. Leptospires colonize the kidneys of their host and can therefore be shed via urine into the environment, contaminating water sources.

  The diagnosis of leptospirosis is difficult in both the clinic and the laboratory and the global disease burden is thus likely underestimated. In Sweden and Denmark, urban rats were identified as a source for leptospirosis with prevalence rates ranging from 24% to up to 89%, respectively, in recent years. It is believed that leptospirosis does not play a role in Norway but currently, no comprehensive data on leptospirosis in Norway are available to support that and the epidemiological situation including potential maintenance hosts is unknown.  

• Aims

  Our network collaborates on different subprojects:

  PhD. student Silje is working on identifying the role of Leptospira in cattle, rodents and shrews in Norway. Moreover, she is studying host-pathogen interactions of Leptospira borgpetersenii serovar Hardjo type hardjobovis in a bovine caruncular epithelial cell line (BCEC-1). Her project will shed light on the current epidemiological situation in Norway and understand the mechanisms that Leptospira use to cause reproductive disease in cattle by harming the placenta.

  Our group linked to the HUNT One Health project aims at screening metagenomic data from livestock and pets for the presence of pathogenic Leptospira spp. and, subsequently, will felect this

• Results/findings

  We have established a qPCR to detect DNA of pathogenic Leptospira species. The qPCR targets a gene encoding outer membrane protein LipL32 and is based on primers and probe described by Stoddard et al. (2013) and Galloway et al. (2015).

• Participants og collaborators

  External participants

  

  Christiane Pfarrer

  Professor, University of Veterinary Medicine Hannover

  Collaborator on Silje Hansen's PhD project

  Anita Haug Haaland

  Collaborator HUNT One Health study

  Collaborators

  The Norwegian Veterinary Institute

  Siv Klevar, researcher and veterinarian

  The Norwegian Institue of Public Health

  • Heidi Elisabeth Heggen Lindstedt, senior advisor, Cand. Agric
  • Arnulf Soleng, Senior researcher

  Institut Pasteur Paris

  Mathieus Picardeau

  Naturformidling van der Kooij

  Jeroen van der Kooij

  Faculty of Chemistry, Biotechnology and Food Science, NMBU

  Bjørn-Arne Lindstedt

Publications

List of publications on Leptospira (co-)authored by the project leader.

Partners in private and public sector

Other relevant projects

HUNT One Health

The HUNT- One Health project is a collaboration between NMBU, the Veterinary Institute and NTNU. The project is managed by the Veterinary Institute and is a sub-project of the population health study in Nord-Trøndelag, HUNT.