BIO211 Laboratory Course in Molecular Biology

The course consists of three independent laboratory assignments. Techniques used

• Polymerase chain reaction (PCR)
• DNA purification
• DNA cloning
• Gel electrophoresis
• Transformation
• Overexpression of recombinant protein
• Protein purification
• DNA and protein sequence analyses
• Mikroskopi

The students write a laboratory report covering

1. Abstract
2. Theoretical introduction
3. Description of methods
4. Presentation of the results
5. Discussion of the results (both positive and negative results)
6. Conclusion

In addition, answers to a set of questions related to the lab exercises is to be attached to the lab report.

Knowledge

Students will gain insight in and hands-on experience with some central technologies in molecular biology, such as PCR, cloning, gel electrophoresis, bioinformatic tools for sequence analyses, purification of DNA and protein, and microscopy. Other central aspects of the course are to learn how to write scientific reports based on experimental data, to participate actively, and to work independently during the course.

Skills

Students can make use of their theoretical knowledge and lab-experience to solve some simple biological problems, such as to use bioinformatic tools to search for sequences, to do sequence analysis, to clone a gene, to control gene expression, to measure biological activity, and to follow and perform protocols for DNA and protein purification and analysis.

The student is able to perform the preparations, techniques and analyses employed in the course. The student masters the instruments and equipment used in the course.

Acquired skills can later contribute to the development of various molecular tools to solve important problems in the society or in the environment. E.g., to

• Improve human and animal health (develop new medicals, vaccines, diagnostic tools, healthier foods etc.).
• Secure life quality on land and in waters by removing toxic pollutants through enzymatic processes,
• Produce more and healthier food using genetic technologies.
• Surveillance and combat/prevent infections in society.

These opportunities and challenges are central in many UN sustainable development goals.

General competence

Students should

• have an overview of and the ability to use central molecular tools in bioresearch.
• be able to work independently in lab
• be able to work in research, on projects with supervision, and
• be able to write a report based on experimental data.